چکیده

Abstract Angiogenesis is involved in several biological processes such as in both physiological and pathological conditions, such as tumor growth and metastasis. VEGFs, including VEGFA, VEGFB, VEGFC, VEGFD, and placental growth factor (PIGF), known as key regulators of both physiological and pathological angiogenesis, and bind to receptor tyrosine kinases (VEGFR-1, VEGFR-2, and VEGFR-3). We have previously reported a peptide (named VGB3) that recognizes both VEGFR1 and VEGFR2, leading to the suppression of intracellular signaling through suppression of PI3K/AKT and MAPK/ERK1/2 signaling pathways, and inhibition of the growth and metastasis of murine 4T1 mammary carcinoma tumors. The main challenge of peptides is that they are small. Human serum albumin (HSA) known as carrier of chemical as well as biological pharmaceutics. Conjugation of peptides to HSA was shown to improve their half-life in circulation. HSA is the most abundant protein in plasma. In the present investigation, HSA was used for conjugation with an antiangiogenic peptide, and the conjugation was assessed using FTIR spectroscopy. Method and Material: Human Serum Albumin 20%, N-(3-dimethy-laminopropyl)-N"-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and anti-angiogenic peptide was prepared. In briefly we dissolved EDC and NHS. Using EDC and NHS to active surface carboxylic acid of HSA. We were analysis the binding peptide and HSA in two difference time and pH and analysis by FTIR spectroscopy and obtained particle were frozen at -30 ̊C lyophilized and stored at 20 ̊C in powder form. Result: FT-IR spectrum was recorded after conjugation of peptide and HSA, and was compared with those from free HSA and peptide. The result showed obvious changes in peaks of conjugated compared to free compounds. We could trace the amide bond, which was stronger in acidic pH and shorter treatment periods. In acidic pH, the peak of amide bond shifted from 1649.49 cm-1 to 1645.53cm-1. Conclusion: These results indicate that HSA nanoparticles could effectively conjugated to the peptide molecules. In addition, we found that the variable of reaction is determinant for the optimal conjugation.

کليدواژه ها

Albumin, Anti angiogenic peptide, Target therapy, FTIR spectroscopy

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سید محسن اصغری , 1400 , تجزیه و تحلیل اتصال پیتید ضد رگ زایی به سرم آلبومین انسانی به وسیله FTIR , نخستین همایش بین المللی و سومین همایش ملی ریاضیات زیستی