چکیده

Background: Recently, Zinc oxide nanoparticles (ZnONPs) synthesized using plants offer immense attention for nanomedicine applications especially in cancer treatments due to their biocompatibility and cost-effective approach. Aim of the study: In the present study, green synthesis of zinc oxide nanoparticles by seed extract of plant Lepidium sativum L. was reported for the first time. And then, we evaluated the cytotoxicity induced by zinc oxide nanoparticles on colorectal cancer (CRC) SW480 cells. Materials and Methods: The physicochemical characteristics of biosynthesized zinc oxide nanoparticles were analyzed by various techniques using UV-visible Spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), Scanning electron microscope (SEM), and energy dispersive X-ray (EDX). Furthermore, cytotoxic activity on SW480 cells was determined by MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay. Results: UV-Visible absorbance spectrum of ZnO nanoparticles showed lambda max at 378 nm while, FTIR studies confirm the presence of functional groups of plant and zinc oxide bond are involved in the formation and stabilization of ZnO nanoparticles. The XRD data indicated the hexagonal phase of the wurtzite structure. SEM analysis exhibits hexagonal shape with size 40.77 nm and EDX spectrum approves the presence of zinc and oxygen. In vitro cytotoxicity studies on SW480 cells showed 50% inhibition growth at concentration of 65.89 µg/ml after 24 h. Conclusions: the outcome of the work proposes that ZnO nanoparticles synthesized from Lepidium sativum L. reveals strong anticancer activity and induce apoptosis on colon cancer cells via stimulating increased oxidative stress. Thus. ZnO nanoparticles might be used as a chemopreventive agent against other cancer cell treatments in the future.

کليدواژه ها

Lepidium sativum L., Green synthesis, Zinc oxide nanoparticles, SW480, Colorectal cancer

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زهره عفتی , 1400 , بررسی سمیت سلولی نانوذرات اکسید روی سنتز شده به روش شیمی سبز , نخستین همایش بین المللی و سومین همایش ملی ریاضیات زیستی