چکیده

Background: Breast cancer is the most common type of cancer among women around the world. Overexpression of the fibroblast growth factor receptor 1 (FGFR1) is a common aberration in many cancers, especially breast cancer. Therefore, FGFR1 is a potential target to treat FGFR1-dependent cancers. Aim of the study: In the present study, an FGFR1 antagonistic peptide was designed to interfere with FGFR1 effects. The potential of the peptide in suppressing bFGF-induced growth was evaluated in 4T1 mammary carcinoma cells by in vitro and in vivo studies. Materials and Methods: An FGFR1 antagonizing peptide was designed based on the structure of bFGF. MTT assay was performed to investigate the effect of the designed peptide on the proliferation of 4T1 mammary carcinoma cells. Furthermore, flow cytometry analysis was done to examine the effect of the peptide on 4T1 cell apoptosis. Finally, the effect of the peptide on tumor growth was investigated using a murine 4T1 tumor model. Statistical analyses and graphs production were performed using Prism software (version 8.0.1 for Windows, GraphPad Software, La Jolla, California, USA; www.graphpad.com), and data were provided as mean ± SEM. Students t-test and unpaired one-way ANOVA supported by Tukey’s post-hoc test were utilized to estimate the significant differences between two and more than two groups. Two-way repeated-measures ANOVA supported by Tukey’s post hoc test was used to estimate the peptide efficacy on the tumor growth. P < 0.05 was considered significant. Results: The results of the MTT assay indicated that the designed peptide could significantly inhibit the bFGF-induced proliferation of 4T1 cells. Moreover, the peptide suppressed anti-apoptotic effects induced by bFGF in 4T1 cells. Also, the peptide effectively inhibited tumor growth in the murine 4T1 tumor model. Conclusions: The results of in vitro and in vivo studies indicate that the antagonistic peptide can effectively inhibit the growth of 4T1 tumors and is a potentially promising agent in treating human breast cancer.

کليدواژه ها

Breast cancer, FGFR1, bFGF, Antagonistic peptide, Tumor suppression

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سید محسن اصغری , 1400 , An FGFR1 antagonistic peptide suppresses the tumor cell growth in vitro and in vivo , نخستین همایش بین المللی و سومین همایش ملی ریاضیات زیستی